GENVEYOR
Horizontal bar chart of gene silencing efficiency normalised to negative control. AccuLNP reaches 0.10, ALC-0315 LNP 0.11 and RNAiMAX 0.11 — all three near-identical and roughly ten-fold below the negative control at 1.00.
Gene silencing efficiency normalised to negative control. 10 nM ALAS1 siRNA delivered by different vehicles: AccuLNP™ 0.10, ALC-0315 LNP 0.11, Lipofectamine RNAiMAX 0.11, negative control 1.00. Lower is stronger knockdown — AccuLNP™ is comparable to both benchmarks.

AccuLNP™ siRNA Transfection Kit

Catalog#
NTP-0300
Size
160 reactions for 24-well plates
Price (USD)
$229

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  • siRNA

Product Overview

AccuLNP-siRNA is a cell transfection reagent mediated by lipid nanoparticles (LNP) for siRNA transfection. AccuLNP can efficiently interact with siRNA molecules to form (lipid molecules + siRNA) complexes and can effectively release siRNA molecules within cells to achieve their biological functions. This reagent has the characteristics of high transfection efficiency, low cytotoxicity, and easy operation.

Product Detail

Catalog# Name Qty
NTP-0300-L Reagent L (liquid) 1 (250 µL)
NTP-0300-A Reagent A (liquid) 1 (250 µL)
NTP-0300-ST Reagent ST (liquid) 1 (10 mL)
Storage condition
-20 °C
Shelf life
12 months
Encapsulation time
~60 s
Cell viability
>90%
Format
Three-reagent liquid kit — Reagent A, Reagent L, Reagent ST

Protocol

Table 1. Recommended conditions for cellular transfection with the AccuLNP-siRNA kit.
For one well 48-well plate 24-well plate 12-well plate 6-well plate
Surface area 1 cm²1.9 cm²3.8 cm²9.6 cm²
Complete growth medium (when plating cells) 250 µL500 µL1 mL2.5 mL
siRNA (10 mM stock) 2.5 pmol — 0.25 µL5 pmol — 0.5 µL10 pmol — 1 µL25 pmol — 2.5 µL
Reagent A 1.625 µL3.25 µL6.5 µL16.25 µL
Reagent L 0.625 µL1.25 µL2.5 µL6.25 µL
Reagent ST (to dilute transfection complex) 25 µL50 µL100 µL250 µL

The following procedure describes how to perform RNA transfections in a 24-well plate. The surface areas of other cell culture vessels are different, and transfection should be scaled accordingly. Follow Table 1 to appropriately increase or decrease the amount of reagent use.

  1. Plate cells

    1. Approximately 18–24 hours before transfection, plate cells in 0.5 mL complete growth cell culture medium per well in a 24-well culture plate. Ideally cells should be at least 70% confluent prior to transfection. For adherent cells, plate at a density of 0.8–3.0 × 10⁵ cells/mL; for suspension cells, 2.5–5.0 × 10⁵ cells/mL.

    2. Incubate the cell culture overnight.

  2. Prepare the transfection complex

    1. Take the transfection kit out of the −20 °C freezer.

    2. Thaw Reagent A and Reagent ST in a 37 °C water bath.

      Note: Ensure Reagent A and Reagent ST are completely thawed and clear transparent solutions are obtained. Apply vortex mixing to the microfuge tubes of Reagent A and Reagent ST to ensure the solutions are homogeneous. Cool Reagent A and Reagent ST down to room temperature before use.

    3. Thaw Reagent L in a 37 °C water bath for at least 10 minutes. Apply vortex mixing to the microfuge tube of Reagent L to ensure a homogeneous solution is obtained. Cool Reagent L down to room temperature before use.

    4. Add 5 pmol (0.5 µL of 10 mM) siRNA stock solution in a sterile tube.

    5. Add 3.25 µL Reagent A. Pipet gently to mix completely.

    6. Add 1.25 µL Reagent L to the above diluted siRNA solution. Use a vortex mixer to mix quickly for at least 15 seconds. The resulting total volume is 5 µL.

    7. Add 50 µL Reagent ST to dilute the above transfection complex. Pipet gently to mix completely.

  3. Cell transfection

    1. Add the transfection complex prepared in Step 2 (total 55 µL) dropwise to different areas of the well.

    2. Gently shake the culture vessel to evenly distribute the transfection complexes.

    3. Continue culturing in the cell culture incubator for the required time (such as 24 hours), and then the user determines the detection method to evaluate the transfection efficiency.

Notes

  1. Unused solutions of Reagent A, Reagent ST and Reagent L can be stored at −20 °C. Thaw and dissolve again in a 37 °C water bath before next use.
  2. The recommended siRNA stock solution to use is 10 mM. If the siRNA stock solution concentration is higher than 10 mM, dilute it with RNase-free water or appropriate buffer first to make sure the siRNA concentration is at 10 mM, and then proceed to prepare the transfection complex.

Ordering

Catalog number NTP-0300. Net 30 on an institutional purchase order. Prices exclude shipping and applicable tax. For scale-up, custom formats, or bulk pricing, email contact@genveyor.com.